Recent Publications

Structural basis for antibody inhibition of flavivirus NS1-triggered endothelial dysfunction
Biering SB & Akey DL, Wong MP, Brown WC, Lo NTN, Puerta-Guardo H, Tramontini Gomes de Sousa F, Wang C, Konwerski JR, Espinosa DA, Bockhaus NJ, Glasner DR, Li J, Blanc SF, Juan EY, Elledge SJ, Mina MJ, Beatty PR, Smith JL, Harris E. (2021)
Science 2021 Jan 8;371(6525):194-200. doi: 10.1126/science.abc0476.
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Zika virus infection enhances future risk of severe dengue disease.
Katzelnick LC, Narvaez C, Arguello S, Lopez Mercado B, Collado D, Ampie O, Elizondo D, Miranda T, Bustos Carillo F, Mercado JC, Latta K, Schiller A, Segovia-Chumbez B, Ojeda S, Sanchez N, Plazaola M, Coloma J, Halloran ME, Premkumar L, Gordon A, Narvaez F, de Silva AM, Kuan G, Balmaseda A, Harris E (2020)
Science 2020 Aug 28;369(6507):1123-1128. doi: 10.1126/science.abb6143.

Characterization of the Type-Specific and Cross-Reactive B Cell Responses Elicited by a Live-Attenuated Tetravalent Dengue Vaccine
Michlmayr D, Andrade P, Nascimento EJM,  Parker A, Narvekar P, Dean HJ, Harris E (2020)
J Infect Dis 2020 Jun 4. doi:10.1093/infdis/jiaa346

Primary and secondary dengue virus infections elicit similar memory B cell responses but breadth to other serotypes and cross-reactivity to Zika virus is higher in secondary dengue. Andrade P, Narvekar P, Montoya M, Michlmayr D, Balmaseda A, Coloma J, Harris E. (2020)
J Infect Dis 2020 Mar 20. doi: 10.1093/infdis/jiaa120

Age-dependent manifestations and case definitions of paediatric Zika: a prospective cohort study.
Burger-Calderon R, Bustos Carrillo F, Gresh L, Ojeda S, Sanchez N, Plazaola M, Katzelnick L, Lopez Mercado B,Carey Monterrey J, Elizondo D, Arguello S, Nuñez A, Gordon A, Balmaseda A, Kuan G, Harris E. (2019)
Lancet Infect Dis. 2019 Dec 20. doi: 10.1016/S1473-3099(19)30547-X
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Tracking the polyclonal neutralizing antibody response to a dengue virus serotype 1 type-specific epitope across two populations in Asia and the Americas
Andrade DV, Warnes C, Young E, Katzelnick LC, Balmaseda A, de Silva AM, Baric RS, Harris E. (2019)
Sci Rep. 2019 Nov 7. doi: 10.1038/s41598-019-52511-z10.1038/s41598-019-52511-z
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Increased serum sialic acid is associated with morbidity and mortality in a murine model of dengue disease.
Espinosa DA, Beatty PR, Puerta-Guardo H, Islam MN, Belisle JT, Perera R, Harris E. (2019)
J Gen Virol. 2019 Sep 17. doi: 10.1099/jgv.0.001319
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Endocytosis of flavivirus NS1 is required for NS1-mediated endothelial hyperpermeability and is abolished by a single N-glycosylation site mutation.
Wang C, Puerta-Guardo H, Biering SB, Glasner DR, Tran EB, Patana M1 Gomberg TA, Malvar C, Lo NTN, Espinosa DA, Harris E. (2019)
PLoS Pathog. 2019 Jul 19. doi: 10.1371/journal.ppat.1007938
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Impact of pre-existing dengue immunity on human antibody and memory B cell responses to Zika.
Andrade P, Gimblet-Ochieng C, Modirian F, Collins M, Cárdenas M, Katzelnick LC, Montoya M, Michlmayr D, Kuan G, Balmaseda A, Coloma J, de Silva AM, Harris E. (2019)
Nature Comm. 2019 Feb 26. doi: 10.1038/s41467-019-08845-3.
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Magnitude and Functionality of the NS1-Specific Antibody Response Elicited by a Live-Attenuated Tetravalent Dengue Vaccine Candidate.
Sharma M, Glasner DR, Watkins H, Puerta-Guardo H, Kassa Y, Egan MA, Dean H, Harris E. (2019)
J. Infect. Dis. 2019 Feb 19. doi:10.1093/infdis/jiz081.
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Flavivirus NS1 triggers tissue-specific vascular endothelial dysfunction reflecting disease tropism.
Puerta-Guardo H, Glasner DR, Espinosa DA, Biering SB, Patana M, Ratnasiri K, Wang C, Beatty PR, Harris E. (2019)
Cell Rep. 2019 Feb 5. doi: 10.1016/j.celrep.2019.01.036
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Epidemiological Evidence for Lineage-Specific Differences in the Risk of Inapparent Chikungunya Virus Infection.
Bustos Carrillo F, Collado D, Sanchez N, Ojeda S, Lopez Mercado B, Burger-Calderon R, Gresh L, Gordon A, Balmaseda A, Kuan G, Harris E. (2019)
J Virology. 2019 Feb 5; 93(4). doi:10.1128/JVI.01622-18
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Prior dengue virus infection and risk of Zika: A pediatric cohort in Nicaragua.
Gordon A, Gresh L, Ojeda S, Katzelnick LC, Sanchez N, Mercado JC, Chowell G, Lopez B, Elizondo D, Coloma J, Burger-Calderon R, Kuan G, Balmaseda A, Harris E. (2019)
PLoS Medicine. 2019 Jan 22; 16(1):e1002726. doi: 10.1371/journal.pmed.1002726
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Cyclic Dinucleotide-Adjuvanted Dengue Virus Nonstructural Protein 1 Induces Protective Antibody and T Cell Responses.
Espinosa DA, Beatty PR, Reiner GL, Sivick KE, Hix Glickman L, Dubensky TW Jr, Harris E. (2019)
J. Immunol. 2019 Jan 18. doi:10.4049/jimmunol.1801323
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Dynamics and determinants of the force of infection of dengue virus from 1994 to 2015 in Managua, Nicaragua.
Katzelnick LC, Ben-Shachar R, Mercado JC, Rodriguez-Barraquer I, Elizondo D, Arguello S, Nuñez A, Ojeda S, Sanchez N, Lopez Mercado B, Gresh L, Burger-Calderon R, Kuan G, Gordon A, Balmaseda A, Harris E. (2018)
PNAS. 2018 Oct 16; 115(42):10762-10767. doi:10.1073/pnas.1809253115.
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Comprehensive innate immune profiling of chikungunya virus infection in pediatric cases.
Michlmayr D, Pak TR, Rahman AH, Amir ED, Kim EY, Kim-Schulze S, Suprun M, Stewart MG, Thomas GP, Balmaseda A, Wang L, Zhu J, Suaréz-Fariñas M, Wolinsky SM, Kasarskis A, Harris E. (2018)
Molec. Syst. Biol. 2018 Aug 27; 14(8):e7862. doi:10.15252/msb.20177862.
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Recent advances in understanding the adaptive immune response to Zika virus and the effect of previous flavivirus exposure.
Andrade DV, Harris E. (2018)
Virus Res. 2018 Aug 2; 254:27-33. doi:10.1016/j.virusres.2017.06.019.
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Longitudinal Analysis of Antibody Cross-neutralization Following Zika Virus and Dengue Virus Infection in Asia and the Americas.
Montoya M, Collins M, Dejnirattisai W, Katzelnick LC, Puerta-Guardo H, Jadi R, Schildhauer S, Supasa P, Vasanawathana S, Malasit P, Mongkolsapaya J, de Silva AD, Tissera H, Balmaseda A, Screaton G, de Silva AM, Harris E. (2018)
J. Infect. Dis. 2018 Jul 13; 218(4):536-545. doi:10.1093/infdis/jiy164.
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Featured Publications

Seroprevalence, risk factor, and spatial analysis of Zika virus infection after the 2016 epidemic in Managua, Nicaragua. 
Zambrana JV, Bustos F, Burger-Calderon R, Collado D, Jairo Sanchez N, Ojeda S, Plazaola M, Lopez B, Arguello S, Elizondo D, Aviles W, Kuan G, Balmaseda A, Gordon A, Harris E. (2018)
Proc. Natl. Acad. Sci. USA. 2018 Sep 11;115(37):9294-9299. doi: 10.1073/pnas.1804672115. Epub 2018 Aug 27.

Comprehensive innate immune profiling of chikungunya virus infection in pediatric cases.
Michlmayr D., Pak T.R., Rahman A.H., Amir E.D., Kim E.Y., Kim-Schulze S., Suprun M., Stewart M.G., Thomas G.P., Balmaseda A., Wang L., Zhu J., Suaréz-Fariñas M., Wolinsky S.M., Kasarskis A., Harris E. (2018)
Mol. Syst. Biol. 2018 Aug 27;14(8):e7862. doi: 10.15252/msb.20177862.

Differences in transmission and disease severity between two successive waves of chikungunya.
Gordon, A., Gresh, L., Ojeda, S., Chowell-Puente, G., Gonzalez, K., Sanchez, N., Saborio, S., Mercado, J.C., Kuan, G., Balmaseda, A., and Harris, E. (2018)
Clin. Infect. Dis. 2018 Apr 25. doi: 10.1093/cid/ciy356. [Epub ahead of print].

Antibody-dependent enhancement of severe dengue disease in humans.
Katzelnick., L. Gresh, L, Halloran, M.E., Mercado, J.C., Kuan, G., Gordon, A., Balmaseda, A., and Harris, E. (2017)
Science. 358(6365):929-932.

Longitudinal analysis of antibody cross-neutralization following Zika and dengue virus infection in Asia and the Americas
Montoya, M., Collins, M., Dejnirattisai, W., Katzelnick, L.C., Puerta-Guardo, H., Jadi, R., Schildhauer, S., Supasa, P., Vasanawathana, S., Malasit, P., Mongkolsapaya, J., de Silva, A.D., Tissera, H., Balmaseda, A., Screaton, G., de Silva, A.M., Harris, E. (2018)
J. Infect. Dis. 218(4):536-545.

CD14+ CD16+ monocytes are the main targets of Zika virus infection in peripheral blood mononuclear cells in a paediatric study in Nicaragua.
Michlmayr, D., Andrade, P., Gonzalez, K., Balmaseda, A., and Harris E. (2017)
Nat Microbiol. 2(11):1462-1470. 2017.

Dengue virus NS1 cytokine-independent vascular leak is dependent on endothelial glycocalyx integrity. 
Glasner, D.R., Ratnasiri, K., Puerta-Guardo, H., Beatty, P.R., and Harris, E. (2017)
PLoS Pathog. 13(11):e1006673.

Analysis of individuals from a dengue-endemic region helps define the footprint and repertoire of antibodies targeting dengue virus 3 type-specific epitopes.
Andrade, D.V., Katzelnick, L.C., Widman, D.G., Balmaseda, A., de Silva A.M., Baric, R.S., and Harris, E. (2017)
mBio. 8(5):e01205-17.

Intrahost selection pressures drive rapid dengue virus microevolution in acute human infections.
Parameswaran, P., Wang, C., Trivedi, S.B., Eswarappa, M., Montoya, M., Balmaseda, A., and Harris, E. (2017)  
Cell Host Microbe. 22(3):400-410.e5.

Zika virus targets different primary human placental cells, suggesting two routes for vertical transmission. 
Tabata, T., Petitt, M., Puerta-Guardo, H., Michlmayr, D., Wang, C., Fang-Hoover, J., Harris, E.* and Pereira, L.* (2016)
Cell Host Microbe. In press (Epub July 18, 2016) S1931-3128(16)30300-6. doi: 10.1016/j.chom.2016.07.002. *Co-corresponding authors. 

Dengue virus NS1 disrupts the endothelial glycocalyx, leading to hyperpermeability. 
Puerta-Guardo, H., Glasner, D.R., and Harris, E. (2016)
PLoS Pathog.12(7):e1005738.

Dengue virus non-structural protein 1 (NS1) triggers vascular leak that can be inhibited by anti-NS1 antibodies. 
Beatty, P.R., Puerta Guardo, H., Killingbeck, S., Glasner, D., Hopkins, K., and Harris, E. (2015)
Sci Transl Med. 7:304ra141.

Evidence based community mobilization for dengue prevention in Nicaragua and Mexico (Camino Verde, the Green Way): cluster randomized controlled trial.
Andersson, N., Nava-Aguilera, E., Arostegui, J., Morales-Perez, A., Suazo-Laguna, H., Legorreta-Soberanis, J., Hernandez-Alvarez, C., Fernandez-Salas, I., Balmaseda, A., Cortés-Guzmán, A.J., Coloma, J., Ledogar, R.J., and Harris, E. (2015)  
BMJ. 351:h3267.